【濃度】:10mg/ml
【儲存條件】：-20℃
【有效期】：12個月
Summary:
Quantity size: 1mg
Purification method: Protein A or Protein G
Purity: >90%
Concentration: 10mg/mL buffer=0.01M PBS (pH7.2-7.4) with 0.03% Proclin300.
Immunogen: IgG
Western blotting: 1:5000-50000
ELISA: 1:10000-100000
ELISA coating: 10-20ug/mL
Immunochromatographic control-line: 2mg/mL
Antibody conjugation: 500ug-20mg/mL
Gold conjugation: Borate buffer (PH=9.0)
HRP conjugation: 0.01mol/L CB (PH=9.0)
Fluorescein conjugation: 0.5mol/L CB (PH=9.5)
Biotin conjugation: 0.1mol/L NaHCO3
Storage: Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).
Background:
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL
in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B
lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to
the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The
variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at
a given time point is estimated to be 1011 variants.
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